An Enhanced Amperometric HER2 Immunosensor for Early Detection of Breast Cancer

Authors

  • Reeti Chaudhary Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India Author
  • Bhawna Nohwal Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India Author
  • Himani Guliya Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India Author
  • CS Pundir Department of Biochemistry, M.D. University, Rohtak-124001, Haryana, India Author

DOI:

https://doi.org/10.47363/JBBER/2025(3)128

Keywords:

HER2, Graphene Sheets, Pencil Graphite Electrode, Immunosensor, Breast Cancer

Abstract

Human epithelium receptor 2(HER2) is the most important breast cancer biomarker, whose increased level in blood is very important for detection of breast cancer at early stage We describe herein the enhancement of an HER2 antibody based amperometric immunosensor for detection of breast cancer, by graphene (Gr) sheets. The working electrode i.e. anti-HER2/Gr-sheets/PGE was characterized using cyclic voltammetry, scanning electron microscopy and electrochemical impedance spectra. The immunosensor had optimum activity at pH 7.0, 25°C, and antibody concentration of 18 µg/ml. It exhibited a linearity in the concentration range 1 fg/ml to 20 ng/ml, with a detection limit (LOD) of 0.85fg/ml. The immunosensor was employed to determine HER2 concentrations in sera of apparently healthy women and those women suffering from breast cancer at early stage. It was increased significantly(p

Author Biographies

  • Reeti Chaudhary, Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India

    Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India

  • Bhawna Nohwal, Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India

    Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India

  • Himani Guliya, Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India

    Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, Haryana, India

  • CS Pundir, Department of Biochemistry, M.D. University, Rohtak-124001, Haryana, India

    Department of Biochemistry, M.D. University, Rohtak-124001, Haryana, India

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Published

2025-03-27

Issue

Vol. 3 No. 2 (2025): Volume 3 Issue 2

Section

Articles