Gene Editing via Integrase Enzyme

Abstract

Targeted integrase enzyme is a most powerful tool for mediating genome alteration with high precision. The gene of the interest is directly catalyze nucleophilic attack of the 3 prime hydroxyl group at the end of processed DNA on a pair of phosphodiester bond in the targeted DNA or genome of the interest. Integrase gene editing method contain two parts gene of the interest with integrase enzyme and desire genome. The integrase enzyme is start integration the gene of the interest into the desire genome or DNA.