The Role of ICAM-1 Expression in Renal Tissue in the Progression of Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus

Authors

  • TS Ryabova Assistant Professor, Doctor of Medical Sciences, Department of Nephrology and Efferent Therapy, S.M. Kirov Military Medical Academy, 194044, Saint Petersburg, St. Academician Lebedeva, 6, Russia Author
  • IA Rakityanskaya Professor, Doctor of Medical Sciences Department of Allergology, Immunology and Clinical Transfusiology, Municipal Outpatient Hospital no. 112, 195427, Saint Petersburg, St. Academician Baykova, 25/1, Russia Author

DOI:

https://doi.org/10.47363/JONE/2024(4)132

Keywords:

Type 2 Diabetes Mellitus, Diabetic Kidney Disease, Intercellular Adhesion Molecule-1 (Icam-1), Renal Tissue Biopsy

Abstract

Introduction: Diabetic nephropathy is the main microvascular complication of diabetes mellitus (DM). Intercellular adhesion molecule 1 (ICAM-1) plays a critical role in the development of morphological changes in renal tissue in patients with type 2 (T2DM) diabetes mellitus. The work is devoted to studying the role of ICAM-1 (CD54) expression in renal tissue in the development and progression of morphological changes in diabetic kidney disease (DKD) in patients with T2DM.
Objective: The examination was carried out in 50 patients with T2DM (mean age 66.58±3.27 years). There were 35 women, 14 men. The duration of disease in patients with diabetes was 17.70 ± 0.35 years. The duration of DKD from the moment of detection of microalbuminuria to the morphological examination of the renal tissue and diagnosis was 1.65±0.34 years.
Methods: All patients underwent light and immunofluorescence microscopy of kidney tissue biopsies. Morphological changes in tissue were assessed according to the latest international classification of diabetic nephropathy, developed in 2010. CD54 (ICAM-1) expression was determined using monoclonal antibodies labeled FITC (anti-human CD54 Antibody) (USA).
Results: According to light microscopy, class IIa (mild mesangial expansion) was detected in 12 patients, class IIb (severe mesangial expansion) in 14 patients, class III (Kimmelstiel-Wilson lesions) in 19 patients and in 5 patients – class IV (advanced diabetic glomerulosclerosis). It has been shown that there is a regression model between the expression of ICAM-1 in the glomerular endothelium with the development of expansion of the mesangial matrix, thickening of the basement membrane and arteriolar hyalinosis. Expression in peritubular capillaries is associated with the development of tubulointerstitial fibrosis and progression of diabetic kidney disease in patients with T2DM.
Conclusions: Based on the data obtained, it was suggested that the study of ICAM-1 expression in kidney tissue can be used as a biomarker for the progression of kidney disease in patients with T2DM.

Author Biographies

  • TS Ryabova, Assistant Professor, Doctor of Medical Sciences, Department of Nephrology and Efferent Therapy, S.M. Kirov Military Medical Academy, 194044, Saint Petersburg, St. Academician Lebedeva, 6, Russia

    Assistant Professor, Doctor of Medical Sciences, Department of Nephrology and Efferent Therapy, S.M. Kirov Military Medical Academy, 194044, Saint Petersburg,
    St. Academician Lebedeva, 6, Russia

  • IA Rakityanskaya, Professor, Doctor of Medical Sciences Department of Allergology, Immunology and Clinical Transfusiology, Municipal Outpatient Hospital no. 112, 195427, Saint Petersburg, St. Academician Baykova, 25/1, Russia

    Professor, Doctor of Medical Sciences Department of Allergology, Immunology and Clinical Transfusiology, Municipal Outpatient Hospital no. 112, 195427, Saint
    Petersburg, St. Academician Baykova, 25/1, Russia

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Published

2025-03-30

Issue

Vol. 4 No. 2 (2024): Volume 4 Issue 2

Section

Articles