Microbial Purification of Oil and Gas Production Water at the Petrochad(mangara) Limited Badila Field

Abstract

RPG14, RPG18 and RPG20 isolates selected at the end of a screening test were subjected to optimization of physicochemical and nutritional parameters. As a result, a 3-liter extraction for each culture medium was launched. The optimal yield after 20 days of incubation is estimated for RPG14 is 68.62 g/l, for RPG18 is 60.42 g/l and for RPG20 is 69.85 g/l. Five graduated tubes containing 150 ml each of oil and gas production water and therefore 25, 30 and 50 ml of supernatant of each isolates RPG14, RPG18 and RPG20 are added to each tube. The whole is placed on an electric centrifuge heater type MPW-260RH and rotates at 300 rpm for 15 minutes. At each centrifugation under a temperature (55, 70 and 75°C), the tubes are passed to a mini-wastewater decanter type 6016-SIMOP. The volume of the oil layer in the tube is read after 20min, 40min and 60min of decantation. The purification rate is calculated. The results reveal that biosurfactants can sanitize the EPP 100%. However, the quantity of biosurfactants does not have an influence on the purification. However, the longer the residence time of biosurfactants in the EPP, the higher the purification rate. The purified EPP is collected and subjected to a physicochemical analysis, these waters comply with the IFC 2007, WHO and FAO discharge standards.