Preparation of Nitric Oxide Releasing Solution and Assessment of in Vitro Efficacy on Sars-Cov-2 Virus Using Plaque Assay

Abstract

In our present research, a method for preparing acidified nitrite solution comprising of biocompatible liquids which is able to release an active ingredient Nitric oxide (NO) gas at a pH scope of 3-4 is revealed. By performing an In-Vitro viral plaque assays, the cytopathic effect of NO on respiratory virus such as SARS-CoV-2 was determined. We discovered that the number of plaques reduced in a dose dependent manner as viral entities were found sensitive towards NO gas. Hence, we assert that our NO releasing acidified nitrite solution is relevant as it has shown a cytopathic effect and inhibited the replication of respiratory viruses like SARS-CoV-2 and other rhinovirus spp. Besides, a convenient and portable delivery system has
been provided for administration of NO gas instead of cylinder-based system.