Bio Nanotube Its Application in Vaccination and Cancer Therapy

Abstract

Microfluidic activities predominate all processing of metal, polymers, Artificial Internal Organ (AIO), Regenerative medicine and Biological systems. Microfluidic system is also used in RT-PCR (Real Time Polymerase Chain Reaction) automatic to use reverse transcriptase, to design C-(Cloned)- DNA to initiate, DNA-DNA or DNA-RNA hybridization to detect/separate corona infected person from non-infected one. BNT (bionano tube) of Escherichia coli was made by isolated fimbriae (pili) of hybrid, genetically engineered) E. coli K-12 C600, Yale USA strain. BNT is made by isolated fimbriae (pili), growing in outermost surface antigen of E. coli and are differentiated by O: K:H: If BNT are immobilized by cancer drugs they could pursue bidirectional AAIR to prevent benign tumor to malignant. This is possible, because GE E. coli is made by the cloning of isolated tumor genes. BNT, if made GE hybrid E. coli, particularly by cancer isolated gene, fimbriae of GE E. coli would response humoral antibodies against that specific cancer cell. Similarly, it would response the AAIR against opportunistic microbial infections. The slow movements of DNA were essential during reverse transcriptase, involved in polymerase chain reaction. Micro fluidic transport supports RT-PCR. Reverse transcriptase enzyme reactions, molecules, convert DNA to RNA, to detect corona/viral m-RNA. The m-RNA moves from spike proteins, to lung alveoli cells by fusion of spike proteins, help to transcribe, translate and multiply corona viral particles and to spread corona. Virulence and pathogens are determined by the adhering nature of microbes and also cancer cells. They are all being characterized by innate immunity, varied from individual to individual and community to community. ETEC (Enterotoxignic-(O8 :) EPEC (Enter pathogenic-, (O26:) and UTI (Urinary Tract Infective) (O25: O:11) Escherichia coli carry plasmids, during their infective/virulent phase. These plasmids were identified in AGE (Agarose Gel Electrophoresis). MRHU (D-mannose Resistant haemagglutination of Human Erythrocytes) plasmids determines the adhering nature of microbes. The HA tests in vitro, virulence test (+/-) vivo in Balb/C mice and plasmid test in AGE established the relations of AAIR All Balb/ C mice vivo phenotypic expressions were made intraperitoneal inoculum. The virulence/adherence / pathogenic E. coli as ETEC, EPEC/UTI were used to design AAIR.