Production and Evaluation of Biodegradable Crab Shell ChitosanNanoparticles

Abstract

Chitosan nanoparticles (CNPs) are potential drug carriers because of their biocompatibility, biodegradability, and functional diversity. The synthesis,
characterization, storage stability, and in vitro release behavior of CNPs were the focus of this research. The morphological characterization of CNPs was conducted through Scanning Electron Microscopy (SEM), which showed spherical particles with a smooth surface and uniform size distribution in the nanometer scale, proving the efficacy of the synthesis procedure. Fourier Transform Infrared (FTIR) spectroscopy was utilized for the detection of functional groups and proving successful interaction between crosslinking agent and chitosan. The FTIR spectra revealed typical chitosan peaks with shifts that confirmed successful crosslinking and incorporation of the drug without any indication of chemical degradation. The in vitro release of the drug was carried out in
phosphate-buffered saline (PBS, pH 7.4) with the dialysis bag technique. The release profile revealed a sustained and controlled drug release over a longer duration, indicating the efficacy of CNPs as effective delivery systems for drugs. Storage stability of CNPs was assessed for 60 days at room temperature and at 4°C. The nanoparticles demonstrated physical stability, size, and drug content without any changes, validating their high storage stability. Generally, the research observed successful preparation of stable, effective CNPs with good drug release profile, validating their applicability in food and pharmacy.