A Smart, Sensitive Estimation of Thiamine Chloride Hydrochloride(Vitamin B1) in Pharmaceutical Preparations and Environmental Wastewater Samples: Application to Content Uniformity TestingA Smart, Sensitive Estimation of Thiamine Chloride Hydrochloride(Vitamin B1) in Pharmaceutical Preparations and EnvironmentalWastewater Samples: Application to Content Uniformity Testing

Abstract

A fast, greener, simple, accurate, precise, rapid, economical and high sensitive ultraviolet spectrophotometric method has been developed for the determination of Thiamine Chloride Hydrochloride (Vitamin B1) in pharmaceutical preparations and environmental wastewater samples, which shows maximum absorbance at 235 nm in distilled water. Beer’s law was obeyed in the range of 2.5-30μg/ ml, with molar absorptivity of 1.028x104 L.mol-1.cm-1, relative standard deviation of the method was less than 1.9%, and accuracy (average recovery %) was 100 ± 0. 8. No interference was observed from common excipients and additives often accompany with Thiamine Chloride Hydrochloride in pharmaceutical preparations. The method was successfully applied to the determination of Thiamine Chloride Hydrochloride in some pharmaceutical formulations (tablets) and industrial wastewater samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of Thiamine Chloride Hydrochloride in true samples.