Characterization And Susceptibility Pattern of Gram Negative Bacteria Isolated From Urinary Tract of Pregnant Woman Attending Antenatal Clinic In Makurdi Metropolis

Abstract

T he study investigated the susceptibility pattern of gram negative bacteria isolated from pregnant women attending antenatal at BishopMurray hospital, Makurdi Benue State Nigeria. A total of 40 clean catch midstream urine samples were collected from the hospital laboratoryin sterile bottles and taken to the school laboratory. Inoculation of the samples to the media was done using the streak method.Twenty eight (28) samples contained gram negative isolates identified by colony morphology, microscopy and biochemical test whichincluded: Escherichia coli 12(30%), Pseudomonas aeruginosa 9(22.2%), and Klebsiella pneumoniae 7(17.5%).The susceptibility of theisolated bacterial strains were tested against 10 different antibiotics using the disc diffusion method and their activity was evaluated byobserving zones of inhibition, measured and recorded. The results showed that E. coli, Pseudomonas aeruginosa and Klebsiella pneumoniawere highly susceptible to ciprofloxacin (with zone inhibition 26.50 mm, 24.50 mm, and 25.00 mm respectively), Augmentin(21.3 %) Tarivid (20.6 %), Streptomycin (14.3 %), Gentamycin(18.0 %), Perfloxacin (10.0 % with Pseudomonas completely resistant),Septrin(15.3 %), Amoxacillin (12.9 % with E. coli completely resistant) Sparfloxacin (11.0 % with Klebsiella resistant). The isolates wereresistant to chloramphenicol with no zone of inhibition.