Development and Validation of UV Spectrophotometric Method for Determination of Lisinopril in Bulk and Pharmaceutical Dosage Forms using Vanillin as Chromogen

Abstract

A simple, sensitive, selective and rapid UV spectrophotometric method was described for the determination of lisinopril in Bulk drug and in dosage forms using Vanillin as the chromogenic agent. The method is based on the condensation reaction between primary aromatic amine group present in lisinopril with aromatic Aldehyde, vanillin to produce an intense yellow colored product. The Resulting Schiff’s base shows an absorption maximum at 395 nm. The different affecting formation and stability of the complex were carefully studied. The reaction was carried out in methanol under heat condition reaction. The method was validated for linearity, range, accuracy and precision. The calibration curve was linear (R2= 0.9996) in the concentration ranges 20-180 μg/ml lisinopril. The limit of detection was found to be = 8.236μg/ml, while the limit of quantification was =24.958 μg/ml. The RSD% values for the three precision levels were (<2). The recoveries (n=3) were 98.3%%, 100.3%% and 100.8%% for 50% ,100% and 150% levels respectively. The method was successfully adopted to determine the content percent of lisinopril in two tablet brands marketed in Sudan.