Changes in the Content of Neuroglobin in the Neurons of the Cerebral Cortex of Rats with Ischemia

Abstract

Introduction: Neuroglobin is a metalloprotein of the family globin, which contains protoporphyrin with an iron atom in the center, forming six coordination bonds. Due to its ability to bind oxygen, Ngb enhances the supply of oxygen to the mitochondria of metabolically active neurons.

Objectives: To assess the content of neuroglobin in the brain of rats with cerebral ischemia of varying severity.

Materials and methods: The experiments were performed on 60 male outbred white rats weighing 260±20 g in compliance with the requirements of the Directive of the European Parliament and of the Council No. 2010/63/EU of September 22, 2010 on the protection of animals used for scientific purposes. Modeling of the cerebral was carried out under conditions of intravenous thiopental anesthesia (40-50 mg/kg). The studies used models of total, subtotal, stepped subtotal, and partial cerebral ischemia.

Conclusion: The most pronounced disorders of the prooxidant-oxidant balance, as well as a decrease in the content of neuroglobin, were observed during cerebral ischemia lasting 1 day. During cerebral ischemia with ligation of both common carotid arteries with an interval of 7 days, an increase in the content of neuroglobin was noted, as a manifestation of the activation of compensatory mechanisms.