Determination of the ED50 of Diarrhea Stop Capsule in Rats

Authors

  • Hussien O Kadi Sanaa University, Faculty of Medicine, Department of Pharmacology & Therapeutics, Sanaa, Yemen Author
  • Ismail Hamoud Ali Al geobri Yemen University, Faculty of Medical Sciences, Sanaa, Yemen Author
  • Abdulmalek Abu Donia Sanaa University, Faculty of Medicine, Department of Pharmacology & Therapeutics, Sanaa, Yemen Author
  • Abdullalh Alharzi Sanaa University, Faculty of Medicine, Central Laboratory for Medical Analysis, Sanaa, Yemen Author
  • Mohammed H Kadi Yemen University, Faculty of Medical Sciences, 48 Hospital, Sanaa, Yemen Author

DOI:

https://doi.org/10.47363/JGHR/2026(7)188

Keywords:

Diarrhea, Stop, Capsule, ED50, Rats

Abstract

Introduction: Diarrhea remains a significant cause of morbidity and mortality globally, particularly in developing countries. The ED50 represents the doseat which 50% of the treated population exhibits a therapeutic response in case cessation of diarrhea.

Objective: The aim of this study was to determine the median effective dose (ED50) of Diarrhea Stop Capsule in albino rats.

Methods: A total of 70 rats were divided into seven groups (n=10/group) and administered increasing oral doses: 25, 50, 75, 100, 125, and 150 mg/kg, withone group as control. Diarrhea was induced by administered 2ml castor oil orally for each rat.

Results: Responses were observed and recorded over 6 hours. The ED50 was calculated using the Probit method. The results show a dose-dependent effect,with an estimated ED50 of 83.4 mg/kg.

Conclusion: The Diarrhea Stop Capsule demonstrated dose-dependent antidiarrheal activity in rat, with an ED₅₀ of approximately 83.4 mg/kg. These results support further pharmacological and clinical studies to standardize dosing protocols for human use.

Author Biographies

  • Hussien O Kadi, Sanaa University, Faculty of Medicine, Department of Pharmacology & Therapeutics, Sanaa, Yemen

    Hussien O kadi, Sanaa University, Faculty of Medicine, Sanaa, Yemen.

  • Ismail Hamoud Ali Al geobri, Yemen University, Faculty of Medical Sciences, Sanaa, Yemen

    Ismail Hamoud Ali Al geobri  Yemen University, Faculty of Medical Sciences, Sanaa, Yemen

  • Abdulmalek Abu Donia, Sanaa University, Faculty of Medicine, Department of Pharmacology & Therapeutics, Sanaa, Yemen

    Abdulmalek Abu Donia Sanaa University, Faculty of Medicine, Department of Pharmacology & Therapeutics, Sanaa, Yemen

  • Abdullalh Alharzi, Sanaa University, Faculty of Medicine, Central Laboratory for Medical Analysis, Sanaa, Yemen

    Abdullalh Alharzi  Sanaa University, Faculty of Medicine, Central Laboratory for Medical Analysis, Sanaa, Yemen

  • Mohammed H Kadi, Yemen University, Faculty of Medical Sciences, 48 Hospital, Sanaa, Yemen

    Mohammed H Kadi  Yemen University, Faculty of Medical Sciences, 48 Hospital, Sanaa, Yemen

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Published

2026-06-27

Issue

Vol. 7 No. 3 (2026): Volume 7 Issue 3

Section

Articles