Early Predictive Biomarkers for Hypertension Using Human FetalAstrocytes

Authors

  • Kaneez Fatima Shad School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia Author
  • Fahmida Abdi School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia Author
  • Ann M Simpson School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia Author

DOI:

https://doi.org/10.47363/JVMS/2024(2)109

Keywords:

Hypertension, Astrocytes, Biomarkers, Glial Fibrillary Acidic Protein (GFAP), Proteomics, Confocal Microscopy

Abstract

Background: Hypertension (HTN) is a major risk factor for Cerebrovascular and cardiovascular diseases, causing premature deaths. Hence, there is an
urgent need for predictive biomarkers to detect HTN before its onset.
Methods
• Cell Culture Technique: Growth of HFAs, followed by conversion of normal (A2) HFAs intoreactive (A1) type, by using ATP solution.
• Confocal Microscopy: Quantification of Glial Fibrillary Acidic Protein (GFAP) in A1 and A2 HFAs, by using antibody ab 7260 with AF 488.
Proteomics Methos
• Extraction of proteins from A1 and A2 HFAs, using Single-pot, Solid-phase-enhanced Sample Preparation (SP3) protocol.
• Liquid Chromatography- Mass Spectrometry techniques for obtaining the lev-els of calcium-gated proteins in A1 and A2 cell-solutions.
Results
• Micrographs obtained by Leica Microsystem indicated thicker filaments in A1 HFAs compared to A2 HFAs.
• Confocal results showed higher levels of GFAP in A1 HFAs (P> 0.05).
• Proteomics data analysed by PEAKS software revealed that proteins, such as GFAP were higher in reactive HFAs.
Conclusions: The results showed higher levels of GFAP in A1 HFAs. Therefore, early detection of GFAP in human body fluids could be a reliable predictive
biomarker for HTN.

Author Biographies

  • Kaneez Fatima Shad, School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia

    School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia

  • Fahmida Abdi, School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia

    School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia

  • Ann M Simpson, School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia

    School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia

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Published

2024-07-30

Issue

Vol. 2 No. 3 (2024): Volume 2 Issue 3

Section

Articles