Discordance in Cytokine Expression -IL-1β and IL-17 in Variety of Disease Including Hepatocellular Carcinoma (HepG2) Cells in Different Culture Conditions

Abstract

The comprehensive study has been designed to carry the immunomodulatory, in the young females (age group-22-28 years) from the blood in the presence post biotic compound designated Z-compound. These healthy female (n= 39) rich in stem-cells also known as Men SCs. Present study compromise five experimental group, and each group consist of (n=9) individuals - 1) morphological analysis of PBMC-MenSCs in co-cultures by fluorescence microscopy, 2) ELISA-based cytokine profiling of IL-1β and IL-17 using monoclonal antibodies, 3) MTT cell viability in hepatocellular carcinoma cells, and 4) isolation and functional characterization of MenSCsderived exosomes with and without Z-compound post biotic treatment. The findings demonstrates that MenSCs in combination with Z-compound significantly suppress the pro-inflammatory cytokine IL-1β (from 0.448 to 0.339 O. D units) and modulate IL-17 levels across three experimental days. Microscopically, PBMC morphology showing notable transformation upon co-culture with MenSCs derived exosomes, with cell diameter increasing from 167 μm to 192.17 μm, consistent with monocyte-to-macrophage (M2 phenotype) differentiation. Exosomes isolated from Z-compound-treated MenSCs exhibited inhibitory properties against cancer cells, while exosomes from non-post-biotic-treated MenSCs demonstrates anti-aging effects due to promoting growth factors and hair follicle stimulation. Preliminary findings become relevant and support the therapeutic adaptability of MenSCs exosome in cancer for regenerative medicine.