Physical Stimualtion for Delivery of Curative Liposome to Dermal Diabetic Tissue

Abstract

Various delivery system were tested as carriers of curative compounds in last 20 years. Subdermal wound tissue and cardiac ischemic tissue are target of many experimental nanoparticles in literature. Our experimental study reports the synthesis of nano-liposomes as vehicles for miR-21, miR-126 and insulin. The liposomes were not injected directly into the diabetic foot tissue in gel or solution, however loaded to special ultrasound compatible gel body and arranged to surface plane of the diabetic foot. The final gel formulation was able to effectively release of containing liposomes to sorounding space of dermal tissue, the specific ultrasound pulses accelerated the liposome release. The transport of the liposomes were visualised using confocal microscopy, the kinetic of lipsomes in real tissue were quantified by CT-tomography. Themethods have potential to be upgraded for delivery  of specific bioactive compounds in cardiology and dermatology