Random UV-Mutagenesis of Lactobacillus Species for the Generation of a Mutant with Better Probiotic Potential

Abstract

Antibiotic resistance has reduced the value of synthetic drugs, offering up possibilities for the development of new substitutes with significant potential to benefit their host. Probiotics are living microbes that restore the microbiome in the gut. The main purpose of this research is to explore the probiotic potential of Lactic Acid Bacteria and its mutant strains. LAB was isolated from a variety of fermented foods. They were first examined for cultural, microscopic, and biochemical properties. The four probiotic LAB isolates were identified to the strain level using 16S rRNA gene sequence comparisons. The tolerance of these strains to pH, NaCl, and phenol was used to establish their safety. In vitro testing for probiotic potential comprised survival under simulated GI tract conditions and antimicrobial activity. Lastly exposure to U.V. for varied periods for strain improvement. The isolates were screened as Limosilactobacillus fermentum (LAB01), Lactobacillus acidophilus (LAB02), Lactobacillus paracasei (LAB03) and Lactobacillus delbrueckii subsp bulgaricus (LAB04). Mutated strains were resistant to a wide spectrum of antibiotics, whereas most strains were only tolerant to limited antibiotics. The correlation between gut flora and health is exciting since it opens new avenues for research. Based on these findings, it can be concluded that LAB is a potent probiotic.