In Vitro Anti-Inflammatory, Antioxidant, Anti-Helminthic and Antimicrobial Activity of Pet. Ether Fractions of the Stem Bark of Macaranga Barteri

Abstract

The stem bark of Macaranga Barteri is used as a worm expellant and gonorrhea management. This research evaluates the bioactivities of ethanolic and petroleum ether fractions of the stem bark of Macaranga Barteri. Standard methods were employed for phytochemical screening. The broth dilution method was utilized in antimicrobial activity. DPPH assay was used for antioxidant activity. In vitro anti-helminthic activity was determined using earthworms (Eudrilus eugeniae). There were forty-eight fractions (W1-W48) and was bulked into W(W1-W8), X(W9-W15), Y(W16-W33) and Z(W34-W48). Glycoside was in petroleum ether fractions for W. Phenolics and saponins were present in fraction X. Steroids, Polyphenols and saponins were in fraction Y. Phenols, steroids, tannins, terpenoid, glycosides, phytosterol, polyphenols and saponins were present in fraction Z. MIC of 12.5±0.017 were obtained for the pet. ether fractions X against Streptococcus aureus. The IC50 of the most potent pet. ether fraction, X and the reference drug. With DPPH scavenging activity were 15.47±0.01 and 51.95±0.01. At 20 µg/ml. However, At 20 µg/mL, the paralysis and death time for both the pet. ether fraction Y and Mebendazole drug were 20.23±15 min, 31.60±18min and 53.06±2.89 min, 82.03±47.37 min. The pet. ether fractions of Macaranga Barteri possess antioxidant, anti-helminthic,
and antimicrobial activities.