Formulation and Evaluation of Methotrexate Nanoparticles byDesolvation Technique

Abstract

Aim: To formulate and evaluate Methotrexate nanoparticles by desolvation technique using intermittent addition method and continuous addition method.

Background: Methotrexate (MTX) is an anti-metabolite commonly utilized in chemotherapy and immunosuppressant in auto-immune diseases. Methotrexateis an FDA-accredited folic acid antagonist indicated for the remedy of rheumatoid arthritis due to its excessive potency and efficacy in such patients. Themedicinal drug is likewise safe and powerful for sufferers with psoriasis, systemic lupus erythematosus, inflammatory bowel disorder, vasculitis, and many different connective tissue sicknesses. Despite the improvement of various newly focused treatment options, MTX stays the spine of RA remedy because of its robust efficacy and tolerability.

Methods: Methotrexate nanoparticles were prepared using desolvation technique. Two formulations were made, in which one formulation was preparedusing intermittent addition method and the other formulation was prepared using continuous addition method. All the formulations were evaluated for drug content, percentage yield and drug release.

Results: Among the prepared two formulations, Formulation prepared using continuous method showed 100% of percentage yield whereas formulationprepared using intermittent showed 97.5 % of percentage yield. The drug content of both formulations showed was respectively 99.2% and 99.8%. Invitro drug release for 6 hrs showed 98.6 and 99.2% drug release.

Conclusion: In this study methotrexate loaded nanoparticles were prepared by intermittent addition method and continuous addition method by usingBovine serum albumin as protein, ethanol and and water as solvents. This study was performed to determine parameters such as percentage yield, drug content and invitro drug release studies. Desolvation technique was considered as the best technique as it shown best and promising results.